国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

簡要描述:CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2280

產(chǎn)品分類

Product Category

詳細(xì)介紹


CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


ATCC® Number:CRL-2268™Price:$329.00
Designations:BE(2)-C

Depositors:JL Biedler

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:neuroblast
CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


Source:Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Restrictions:BE(2)-C was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. BE(2)C is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)BE(2)-C or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of BE(2)-C including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:Isolation date: November, 1972

DNA Profile (STR):Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 6

TPOX: 11

vWA: 18



Age:2 years

Gender:male

Comments:BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

BE(2)-C cells have a reported saturation density of greater than 5 X 10 exp5 cells/sq cm.

The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).

Unlike the parent line, they generally do not detach and float.



Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Doubling Time:18 hrs

Related Products:recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2271























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本精品videosse×少妇| 99热最新成人国产精品| 高清播放器欧美大片| 最近2019年日本中文字幕免费| 中文字幕 一区二区三区| 欧美乱妇高清无乱码在线观看 | 99久久国产宗和精品1上映| 午夜精品乱人伦小说区| 野花视频在线观看免费| 国产乱人伦偷精品视频免下载 | 人妻无码av久久一二三区| 日韩精品一区二区三区| 无码人妻一区二区三区色欲av| 精品无码久久久久国产| 精品久久久久久中文字幕| 亚洲国产精品久久久久久无码| 另类SM一区二区三区免费视频| 亚无码乱人伦一区二区| 亚洲精品美女久久7777777| 玩弄放荡人妻一区二区三区| 国产AV一区二区| 曰本无码人妻丰满熟妇啪啪| 久久精品无码精品免费专区| 久久精品中文字幕第一页| 绝伦の上司に一晚人妻| 亚洲AV无码专区亚洲AV伊甸园| 亚洲va国产va天堂va久久| 国产黄在线观看免费观看不卡| 一本大道熟女人妻中文字幕在线 | 亚洲成A人一区二区三区| 免费萌白酱国产一区二区三区 | 国产精品扒开腿做爽爽的视频 | 在免费jizzjizz在线播放| 国产97在线 | 免费| 一本大道精品成人免费视频| 无人在线观看高清免费完整版| 久久婷婷五月综合国产| 少妇被又大又粗又爽毛片久久黑人 | 久久国产人妻一区二区| 人妻聚色窝窝人体www一区| 亚洲AV永久中文无码精品综合|