国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物上皮細(xì)胞>CCL-64Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

簡要描述:CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號:CCL-64
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2923

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞 的詳細(xì)介紹

CCL-64

ATCC® Number:CCL-64™  Price:$275.00
Designations:Mv 1 Lu (NBL-7)

Depositors:AJ Kniazeff

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Musa vison (mink)

Morphology:epithelial


Source:Organ: lung

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: May, 1964

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:adenovirus 5; coxsackievirus A9, B5; poliovirus 2

Cytogenetic Analysis:Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.

Age:near term fetus

Gender:male and female mixed

Comments:The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.The cells are useful for focus forming assays for murine and feline sarcoma viruses [PubMed: 4366800].

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

purified DNA:ATCC CCL-64D

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国内精品伊人久久久久AV| 精品无码av无码免费专区| 久久www免费人成人片| 精品国产黑色丝袜高跟鞋| 在线观看大片免费播放器| 国产成人精品久久久久| 亚洲熟妇色XXXXX欧美老妇Y| 国产成人精品一区二区A片带套| 欧美人妻精品一区二区三区| 无码少妇一区二区三区| 97成人无码免费一区二区中文| 无遮挡国产高潮视频免费观看| 亚洲人妻| 亚洲成av人片天堂网| 老熟妇仑乱一区二区视頻| 久久夜色撩人精品国产小说| 亚洲av一区二区无码波多野结衣| 高傲人妻女教师的沉沦长篇| 国产成人无码综合亚洲日韩| 久久亚洲精品AB无码播放| 中文字幕AV一区| 国产精品久久久久9999| 久久久96人妻无码精品蜜桃| 午夜精品久久久久久99热| 久久女婷五月综合色啪小说 | 久久久久久国产A免费观看| 国产成人无码精品久久久| 永久免费观看不收费的软件5| 国产成人精品综合在线观看| 国产精品污WWW一区二区三区| 国产精品无码免费专区午夜| 久久这里只有精品无码3d| 久久碰国产一区二区三区| 无码人妻久久一区二区三区| 丁香激情综合久久伊人久久| 色妞av永久一区二区国产av| 国产精品毛片大码女人| 欧美精品久久久久a片色戒| 亚洲AV成人一区二区三区AV| 国产AV国片精品无套内谢无码 | 精品人妻人人做人人爽|