国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>HIT-T15 大鼠胰腺B細(xì)胞

HIT-T15 大鼠胰腺B細(xì)胞

簡要描述:HIT-T15 大鼠胰腺B細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:1994

產(chǎn)品分類

Product Category

詳細(xì)介紹

HIT-T15 大鼠胰腺B細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件


HIT-T15 大鼠胰腺B細(xì)胞

ATCC® Number: CRL-1777™    Price: $289.00
Designations: HIT-T15

Depositors: R Santerre

Biosafety Level:2 [Cells Contain PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mesocricetus auratus (hamster, Syrian golden)

Morphology:epithelial
HIT-T15 大鼠胰腺B細(xì)胞


Source:Organ: pancreas
Tissue: islet of Langerhans
Cell Type: beta cell;


Cellular Products:insulin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:glucagon; somatostatin; glucocorticoid

Comments:This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.

The cells secrete up to 24 ng of insulin per 10 exp6 cells per 24 hours (passage 60).

Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.

Insulin synthesis decreases with length of time in culture.



Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
高清欧美性猛交XXXX黑人猛交| 最近免费中文字幕大全高清mv| 国产xxx69麻豆国语对白| 欧洲亚洲精品a片久久99果冻| 9I精品福利一区二区三区蜜桃| 欧美丰满熟妇XXXX喷水| 香港经典a毛片免费观看| 久久久久亚洲精品无码网址色欲| 亚洲精品乱码久久久久久按摩| 免费无码又爽又刺激高潮的app| 色偷偷888欧美精品久久久| 欧美日韩免费观看| 中文无码精品a∨在线| 寂寞少妇扒开双腿猛烈进入免费看| 精品乱人乱XXXX国语对白| 亚洲一区精品二人人爽久久| 色噜噜AV亚洲色一区二区| 99热久久精里都是精品6| 国内大量揄拍人妻精品視頻| 亚洲国产综合久久久精品| 久久久久亚洲av无码专区电影| 人妻人人澡人人添人人爽| 久久天天躁狠狠躁夜夜躁2014| 欧美av蜜桃一区二区蜜桃| А√天堂中文在线资源库免费观看| 久久久久久久97| 无码人妻精品一区二区蜜桃色| 国偷自产一区二区免费视频 | 国产成人一区二区三区| 精人妻无码一区二区三区| 亚洲 日韩 欧美 综合 热| 国内精品久久久人妻中文字幕| 男女猛烈激情xx00免费视频| 久久99精品久久只有精品| 亚洲国产成人片在线观看| 麻豆亚洲av熟女国产一区二| 久久久久久久99精品免费观看| 亚洲av无码国产精品麻豆天美 | 国产精品 高清 尿 小便 嘘嘘| 亚洲国产精品无码专区网站| 国产做A爰片久久毛片A片软件|