国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

簡要描述:CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2075

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞 的詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

ATCC® Number:CRL-1715™  Price:$329.00
Designations:TM4

Depositors:JP Mather

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: testis
Disease: normal
Cell Type: Sertoli cell;


Cellular Products:retinol binding protein

tissue plasminogen activator

transferrin



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:follicle stimulating hormone (FSH), expressed

androgen receptor, expressed

progesterone receptor, expressed



Tumorigenic:No

Antigen Expression:H-Y antigen; Mus musculus, expressed

Age:11 to 13 days

Gender:male

Comments:The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell supension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days


Preservation:Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040



References:1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197

26150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
天黑黑影院在线观看视频高清免费| 无码熟妇人妻av在线网站| 免费看小12萝裸体视频国产| 人妻精品久久久久中文字幕一冢本| 亚洲精品成人片在线观看| 亚洲熟女色情网中文字幕| 人妻少妇乱子伦精品无码专区| 国产农妇精品xxx| 国产AV旗袍丝袜精品无码麻豆 | 亚洲精品久久久无码大桥未久| 最近免费中文字幕大全高清mv| 中文字幕乱码亚洲∧V日本1 | 日本爽快片18禁片免费久久| 护士人妻hd中文字幕| 精品国产av 无码一区二区三区 | 国产精品美女午夜爽爽爽免费| 亲胸揉胸膜下刺激娇喘视频免费| 欧美疯狂XXXXBBBB喷潮| 国内精品乱码卡一卡2卡三卡 | 24小时日本在线www免费的| 亚洲第一网色综合久久红第一 | 国产精品日产欧美久久久| 成人免费电影网站| 东京热av人妻无码a片| 色婷婷7777免费视频在线观看| 国产精品热久久高潮AV| 久久亚洲精品成人AV无码网站| 免费高清视频免费观看 | 竹菊成AV人无码亚洲成AV无码| 欧美毛片免费观看| 中文有无人妻VS无码人妻激烈 | 国产精品亚洲一区二区无码| 国产精品沙发午睡系列99| 国产激情久久久久99蜜桃| 人人妻人人澡人人爽精品欧美| 久久人妻内射无码一区三区| 亚洲综合色视频在线观看| 午夜福利麻豆国产精品| 爆乳熟妇一区二区三区霸乳| 久久精品国产亚洲av网站| 无遮无挡爽爽免费毛片|