国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>Panc 03.27 人胰腺癌細(xì)胞

Panc 03.27 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2549 Panc 03.27
人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
Panc 03.27 人胰腺癌細(xì)胞

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1821

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

ATCC® Number: CRL-2549™ Price: $417.00

Designations: Panc 03.27

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras + (wild-type)

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 8,13

THO1: 6

TPOX: 8,9

vWA: 16,17

Age: 65 years

Gender: female

Ethnicity: White

Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37.5 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


CRL-2549





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
狠狠躁夜夜躁人人躁婷婷视频| 玖玖热麻豆国产精品视频| 亚洲精品巨爆乳无码大乳巨 | 色欲久久久天天天综合网精品| 国产97在线 | 免费| 边摸边吃奶边做爽免费视频99| 一本久久知道综合久久| 400部精品国偷自产在线| 最好免费高清视频在线观看 | md传媒破解版app免费版| 国产亚洲精品久久久久久久软件| 最新69成人国产精品视频免费| 亚洲综合AV一区二区三区不卡| 中文字幕人妻无码乱精品| 无码一区二区三区| 欧美性猛交 XXXX 乱大交| 精品国产制服丝袜高跟| 女人高潮久久久久久久| 亚洲AV无码国产精品色午友在线| 新婚之夜玩弄人妻系列| 成人免费电影网站| 97高清视频在线观看免费| 18vide0sex性欧美| 精品香蕉久久久午夜福利| 黄页网站免费频道大全| 精品久久久久久无码人妻热 | 色 综合 欧美 亚洲 国产| 99久久99久久精品国产片| 成人国内精品久久久久一区 | 无人在线直播免费观看| 国产午夜精品AV一区二区| 国产精品jizz在线观看| 亚洲性色精品一区二区在线 | 免费一对一真人视频app| 日产精品一区二区| 野花免费社区在线| 欧美老妇与zozoz0交| 日韩精品久久久久久久电影蜜臀| 少女动漫在线高清免费观看| 亚洲av无码一区二区三区四区| 国产精品久久久久久AV|