国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-2549Panc 03.27細胞, 人胰腺癌細胞

Panc 03.27細胞, 人胰腺癌細胞

簡要描述:Panc 03.27細胞, 人胰腺癌細胞
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2549
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1379

產(chǎn)品分類

Product Category

詳細介紹

Panc 03.27細胞, 人胰腺癌細胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細胞, 人胰腺癌細胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久亚洲AV无码国产精品| 熟妇人妻videos| 亚洲AV人无码激艳猛片服务器| 久久精品国产网红主播| 大乳丰满人妻中文字幕日本| 少妇人妻无码永久免费视频| julia ann 精品艳妇| 99精品久久久中文字幕| 亚洲欧美婷婷五月色综合| 人人澡人人澡人人看添av| 99草草国产熟女视频在线| 蜜桃精品免费久久久久影院| 无码AⅤ免费中文字幕久久| 亚洲日本va午夜在线电影| 蜜桃影片在线播放网站免费观看| 无人在线观看高清免费完整版| 少妇人妻好深太紧了a片| 日产精品一线二线三线芒果| 欧美老妇激情xxxxxx按摩| 久久久久久久久无码精品亚洲日韩| 无码精品一区二区三区免费视频| 久久黄色网站| 朋友人妻翘臀迎接粗大撞击| 国产熟女高潮视频| free性欧美极度另类| 97人妻一区二区精品免费| 在线a级毛片免费观看| 欧美毛片又粗又长又大电影| 高清一区二区三区日本| 麻豆一区二区三区精品视频| 国产边打电话边被躁视频| 蜜臀AV人妻国产精品李丽| 99re在线精品国产欧美| WWW亚洲精品少妇裸乳一区二区 | 久久人人添人人爽添人人片牛牛 | 98国产精品综合一区二区三区| 日韩一区二区三区精品| 无码熟妇人妻av在线影片软件| 亚洲色婷婷一区二区三区| 国产精品国产三级国产专区53| 麻豆人妻少妇精品无码专区 |