国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2149SK-N-DZ細(xì)胞, 人成神經(jīng)瘤細(xì)胞-骨髓

SK-N-DZ細(xì)胞, 人成神經(jīng)瘤細(xì)胞-骨髓

簡(jiǎn)要描述:SK-N-DZ細(xì)胞, 人成神經(jīng)瘤細(xì)胞-骨髓
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2149
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2024-11-21
  • 訪(fǎng)  問(wèn)  量:1840

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

SK-N-DZ細(xì)胞, 人成神經(jīng)瘤細(xì)胞-骨髓

ATCC® Number:CRL-2149™    Price:$338.00
Designations:SK-N-DZDepositors:C HelsonBiosafety Level:1Shim & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)pped:frozenMediuMorphology:epithelial Source:Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow Cell Type: neuroblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.SK-N-DZ細(xì)胞, 人成神經(jīng)瘤細(xì)胞-骨髓Tumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,11 D5S818: 12 D7S820: 12,13 THO1: 6,9 TPOX: 8 vWA: 16,18Cytogenetic Analysis:modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seenAge:2 yearsGender:femaleEthnicity:CaucasianComments:SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. Retinoic acid induces differentiation in this line. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression.Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 661079222439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 841782423127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694Related Links NCBI Entrez SearchMake a DepositFrequently Asked QuestionsMaterial Transfer AgreementTechnical SupportRelated Cell Culture Products

ATCC® Number: CRL-2149™ Price: $338.00
Designations: SK-N-DZ
Depositors: C Helson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: brain
Disease: neuroblastoma
Derived from metastatic site: bone marrow
Cell Type: neuroblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen
Age: 2 years
Gender: female
Ethnicity: Caucasian
Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.
Retinoic acid induces differentiation in this line.
Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.
Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.
The cells exhibit moderate MDR1 expression.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792
22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824
23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694
Related Links
NCBI Entrez Search
Make a Deposit
Frequently Asked Questions
Material Transfer Agreement
Technical Support
Related Cell Culture Products

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产成人无码一区二区三区| 精品亚洲一区二区三区在线观看| 精品少妇人妻AV一区二区| 欧美精产国品一二三产品区别| 日本一欧美一欧美一亚洲| 亚洲精品第一国产综合野草社区| 中文字幕aⅴ人妻一区二区| 国产人妻精品无码av在线| 国产精品无码一区二区av蜜桃| 国产成人精品一区二区三区| 3D动漫精品啪啪一区二区免费 | 无码熟妇人妻av在线影片软件| 亚洲AV片不卡无码久久| 无码88AⅤ欧美熟妇人妻影院| 亚洲精品无码久久久影院相关影片| 久久国产亚洲精品无码| 国精品人妻无码一区二区三区蜜柚| 成人毛片18女人毛片免费看网站| 亚洲A片无码精品毛片色戒| 久久精品国产精品亚洲蜜月| 亚洲中文字幕无码av永久| 国产精品天堂avav在线| 西瓜视频高清在线观看免费| 亚洲国产精品无码久久一区二区| 伊人久久丁香色婷婷啪啪| 寂寞人妻瑜伽被教练日| 久久人人妻人人爽人人爽| 亚洲精品伦理熟女国产一区二区| 国产SUV精二区九色| 久久国产avjust麻豆| 国精产品一区二区三区有限公司 | 天天夜夜草草久久伊人69堂| 粗大挺进朋友人妻淑娟| 国产婷婷成人久久av免费高清| 亚洲一区精品二人人爽久久| 朋友销魂的人妻| 国产精品久久久久久久久久久| 久久亚洲AV成人无码国产| 亚洲AV色香蕉一区二区三区蜜桃 | 久久亚洲精品久久国产一区二区 | 人人澡人人澡人人看添av|