国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-19BT-20細(xì)胞, 人乳癌細(xì)胞

BT-20細(xì)胞, 人乳癌細(xì)胞

簡要描述:BT-20細(xì)胞, 人乳癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:HTB-19
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1684

產(chǎn)品分類

Product Category

詳細(xì)介紹

BT-20細(xì)胞, 人乳癌細(xì)胞

ATCC® Number:HTB-19™ Price:
Designations:BT-20Depositors:EY LasfarguesBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial Source:Organ: mammary gland; breast Disease: carcinomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Isolation:Isolation date: 1958Applications:BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5. This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and c*tion of cells spilling out of the tumor when it was cut in thin slices.Tumorigenic:YesBT-20細(xì)胞, 人乳癌細(xì)胞Reverse Transcript:negativeAntigen Expression:HLA A1, Bw16 (+/-)DNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 11,14 D5S818: 12 D7S820: 10 THO1: 7,9.3 TPOX: 11 vWA: 16,17Cytogenetic Analysis:Normal chromosomes N3, N4, N9, N13, N14, and X may be absent. The markers der(11)t(11;?)(q25;?) (M1); der(1)t(1;3)(p22?;p13?) (M2); and der(2)t(2;?) (q37;?) (M5) were detected by W.A. Nelson-Rees, et al., Int. J. Cancer 16: 74-85, 1975.Isoenzymes:AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1Age:74 yearsGender:femaleEthnicity:CaucasianComments:The cells express the WNT3 and the WNT7B oncogenes [PubMed: 8168088]. This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and c*tion of cells spilling out of the tumor when it was cut in thin slices. A mycoplasma contaminant was discovered and eliminated early in 1972. Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha). BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311122536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 83387123079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 233490323110: Castles CG, et al. Expression of a constitutively active estrogen receptor variant in the estrogen receptor-negative BT-20 human breast cancer cell line. Cancer Res. 53: 5934-5939, 1993. PubMed: 826140623113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed:816808823212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 1361153723226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 701721232275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 939376432488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品国产精品青草| 精品无码一区二区三区在线| 波多野结衣 美乳人妻| 成全视频在线观看免费高清| 久久久久国产精品嫩草影院欧洲| 99久久国产露脸精品竹菊传媒 | 日本伊人精品一区二区三区| 久久超级碰碰人妻人人妻| 久久久久久伊人高潮影院| 无码精品黑人一区二区三区| 日本三级丰满邻居人妻视频| 欧美精品久久久久a片| 久久99精品国产麻豆不卡| 麻豆av一区二区三区久久| 囯产极品美女高潮无套久久久| 成人无码H免费动漫在线观看| 亚洲视频一区| 欧美人c交zoozooxx| 伊人久久大香线蕉亚洲| 亚洲成av人影片在线观看| 亚洲精品久久久久中文字幕二区| 亚洲av无码乱码精品国产福利| 最近中文字幕在线看免费完整版| 亚洲av永久无码精品| 欧美日本精品一区二区三区| 成人欧美尽粗二区三区AV| 777久久精品一区二区三区无码| 久久精品国产精品亚洲毛片| 久久天堂AV综合合色| 成年轻人电影免费20岁| 性裸交A片一区二区三区| 国产成人亚洲精品无码| 麻豆成人久久精品二区三区免费| 亚洲AV日韩精品久久久久久久| 97精品国产一区二区三区| 亚洲精华国产精华精华液网站| 亚洲AV国产国产久青草| 久久综合九色综合欧美狠狠| 国产精品无码翘臀在线观看| 久久久久99精品成人片直播| 国产精品久久久久jk制服|