国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1948

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03 (ATCC® CRL-2555™)

Organism  Homo sapiens, human

Tissue  pancreas

Product Format  frozen

Morphology  epithelial

Culture Properties  adherent

Biosafety Level  1

Disease  adenocarcinoma

Age  70 years adult

Gender  male

Ethnicity  White

Storage Conditions  liquid nitrogen vapor phase

Derivation  Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Clinical Data  70 years

Caucasian

male

Antigen Expression  MHC class I +; MHC class II -

RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Blood type O; Rh+

Oncogene  K-ras + RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Genes Expressed  cytokeratins 7 and 18 RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Tumorigenic  Yes

Effects  Yes, forms tumors in nude or SCID mice

Comments  The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

The cells have a reported plating efficiency of 80%. Ref

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

•20 Units/ml human recombinant insulin

•fetal bovine serum to a final concentration of 15%


Subculturing  Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: 1:2 to 1:3

Medium Renewal: Add media once per week. Fluid change one to two times per week.


Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation  Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions  Temperature: 37°C




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美老肥妇做爰BBWW| 国产JIZZJIZZ全部免费看| 国产欧美日韩一区二区加勒比| 国产在线拍揄自揄视精品| 久久精品国产亚洲AV麻豆长发| 少妇高潮惨叫久久久久久 | 国产欧美日韩| 人妻无码熟妇乱又伦精品 | 绝伦の上司に一晚人妻| 亚洲日本va午夜在线电影| 久久免费看少妇高潮a片特无毒| 国产精品久久久久AAAA| 亚洲av无码一区东京热| 日韩无码专区| 亚洲日韩精品无码一区二区三区| 日韩精品无码人妻免费视频| 秋霞在线看片无码免费| 精品国产乱码久久久EA7| 秋霞成人午夜鲁丝一区二区三区| 久久久久99精品成人片欧美| 国产AV国产AV在在免费线 | 国产肥熟女视频一区二区三区 | 国产三级在线观看完整版| 欧美ZOOZZOOZ性欧美| 国产午夜福利视频在线观看| 每晚都被他添的好多水免费视频 | 3d动漫精品啪啪一区二区| av无码免费岛国动作片| 在线免费看电影| 国产一区日韩二区欧美三区| 亚洲老熟女AV一区二区在线播放 | 四虎影视久久久免费| 国产伦精品一区二区三区妓女| 亚洲AV无码99久久久国精| 9孩岁女精品╳片| 国产精品久久久久久亚洲| 亚洲精品无码葡京av天堂| 久久香蕉国产线熟妇人妻| 影音先锋中文字幕人妻| 久久亚洲色WWW成人欧美| 男女啪啪久久精品亚洲A|