国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當前位置:首頁  >  技術文章  >  TOV-21G 人卵巢癌細胞

TOV-21G 人卵巢癌細胞

更新時間:2016-08-01      點擊次數(shù):2515
Designations:TOV-21G
Depositors: University of Montreal
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens deposited as human
Morphology:epithelial
 
Source:Organ: ovary 
Tumor Stage: grade 3, stage III 
Disease: primary malignant adenocarcinoma; clear cell carcinoma
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Tumorigenic:Yes
Oncogene:p53 + (wild type)
DNA Profile (STR):Amelogenin: X 
CSF1PO: 13,15 
D13S317: 11,12 
D16S539: 10,12 
D5S818: 12,13 
D7S820: 12 
THO1: 7,9.3 
TPOX: 8,11 
vWA: 17
Cytogenetic Analysis:47, XX, +10 [49408 ]
Age:62 years
Gender:female
Comments:This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended 
Medium Renewal: Every 3 to 4 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

CoC1人卵巢癌細胞系Lec1倉鼠卵巢細胞
A2780人卵巢癌細胞OVAC卵巢癌細胞
Anglne人卵巢癌細胞系NIH:OVCAR-3人卵巢癌細胞
Caov-3人乳突狀卵巢腺癌細胞PA-1人卵巢畸胎瘤細胞
CHO中國倉鼠卵巢細胞Pr-HNV8菜青蟲卵巢細胞
CHO/dhFr-倉鼠卵巢細胞,二氫葉酸還原酶缺陷RP70-a-CHO-0.8MTX中國倉鼠卵巢細胞
CHO-K1中國倉鼠卵巢細胞SF9晚秋粒蟲卵巢細胞
CHO-pt轉(zhuǎn)血小板基因倉鼠卵巢細胞SKOV3人卵巢癌細胞
CHO-S中國倉鼠卵巢懸浮細胞SKOV-3/DDP卵巢癌順鉑耐藥株
CoC1人卵巢癌細胞系SW1910人卵巢癌細胞
CoC2 卵巢癌 SW626人卵巢腺癌細胞
CTLA4 Ig-24中國倉鼠卵巢細胞TOV-21G人卵巢癌
ES-2人卵巢透明細胞癌ZA1轉(zhuǎn)S9基因倉鼠卵巢細胞
HO-8910人卵巢癌細胞ZA6轉(zhuǎn)S9基因倉鼠卵巢細胞
HO-8910PM人高轉(zhuǎn)移卵巢癌細胞 Cho-K1倉鼠卵巢細胞亞株
HOC1卵巢癌細胞SK-OV-3人卵巢癌細胞
Lec1倉鼠卵巢細胞Sf9昆蟲卵巢細胞
CHO-K1倉鼠卵巢細胞亞株ES-2人卵巢透明細胞癌
CHO/dhFr-倉鼠卵巢細胞,二氫葉酸還原酶缺陷SK-OV-3人卵巢癌細胞TOV-21G 人卵巢癌細胞TOV-21G 人卵巢
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
永久免费不卡在线观看黄网站| 国产精品第一区揄拍无码| 777久久人妻少妇嫩草AV| 中文无码熟妇人妻av在线| 免费无码黄十八禁网站在线观看| 亚洲 欧美精品SUV| 久久久精品人妻无码专区不卡| 久久久久久精品天堂无码中文字幕 | 日韩精品内射视频免费观看| 久久99国产精品成人| 亚洲精品卡一卡2卡3卡4卡| 日本熟妇色xxxxx日本免费看| 野花日本韩国视频免费8| 久久久噜噜噜久久熟女AA片| 亚洲一区二区女搞男| 国内永久免费crm系统| 末成年女av片一区二区| 一区二区三区高清不卡视频| 国产偷窥熟女精品视频大全| 97久久精品亚洲中文字幕无码| 国产精品久久久久久久久KTV | 国产精品成人无码视频| 免费无码AV片在线观看播放| 精品人伦一区二区三区蜜桃免费| 欧美a级suv大全| 国产免费午夜A无码V视频| 成人无码H免费动漫在线观看| 欧洲精品99网yours| 中文无码人妻在线一区不卡| 亚洲av无码一区二区乱子仑| 奇米777四色777欧美在线| 免费网站看av片| 蜜芽VA亚洲VA欧美VA天堂| 成人性生交大片免费看中文| 久久久久日本精品无码天美传媒 | 初尝人妻少妇小军| 成全视频在线观看免费高清| 少妇人妻偷人精品无码视频| 国产熟妇无码A片AAA毛片视频| 最近中文字幕大全免费版在线| 疯狂三人交性欧美|